Reticulin is a normal component of the bone marrow stroma and can be detected with a reticulin stain in 73% to 81% of healthy subjects. Reticulin and cd34 stains for the liver core biopsy of a 55year old patient with history of cirrhosis and well differentiated hepatocellular carcinoma hcc. The method developed by gordon and sweet made further improvement on the reticulin silver stain. Pdf refrigerated silver solution is as good as fresh preparation. Reticulin stain kit is a metal impregnation technique where ammoniacal silver. Reticulin stain demonstrates fibers around groups of granulosa cells, while surrounding individual theca cells. Evaluation of bone marrow reticulin formation in chronic. Special stains, such as reticulin stain and cd34 immunostain, are very helpful in the diagnosis of well differentiated hepatocellular carcinoma hcc. The tissue is first oxidized, sensitized with iron alum, which is replaced by a silver salt. Reticulin fibrosis in the bone marrow reticulin stain, openi. Haematopoietic abnormalities associated with tacrolimus are relatively rare with reversible pure red cell aplasia being the most common. We report here two cases of well differentiated hcc with an. Gomoris onestep trichrome staining procedure combines the plasma stain chromotrope 2r and connective fiber stain aniline. Dr lal pathlabs offers test service for histopathology special stain reticulin test for checking surgical pathology.
Recent advances in the pathology of pituitary adenomas. This file is licensed under the creative commons attribution 2. The manuals for this section have been compiled by susan l. Gordon and sweets reticulin histotechnology group dst. Often not helpful in nonclassic cases outlines architecture of liver and spleen to diagnose hemangiopericytoma, vascular smooth muscle, fibrosarcoma or fibrothecoma stains each cell vs. Optic nerve gliomas are rare tumors, accounting for 1. This 10 component comprehensive reticulin stain kit is excellent stain for the identification of reticulin fiber in tissue sections. The fluid in the follicular spaces is mucicarminepositive in twothirds of cases.
Reticulin fibers are argyrophilic, meaning that these tissue elements will stain black with a silver solution using the aid of a chemical reducer which brings the silver into to a visible form. When paraffin sections are mounted on slides and viewed down a microscope, they appear colourless and offer little in the way of information. An intro to routine and special staining in histopathology. Jan 06, 2020 gomoris silver impregnation staining technique for reticulin fibers by editorial team on january 6, 2020 in histopathology reticular fibre is the type of connective tissue composed of type iii collagen secreted by reticular cells. Unusual reticulin staining pattern in welldifferentiated hepatocellular carcinoma by heng hong, bryan patonay and james finley topics. Gomori reticular fiber stain research 042016 powerpoint. The quantitative and qualitative parameters of reticulin fibers identified in 25 bm biopsies 67. Ferric ammonium sulfate acts as sensitizer, replaced by silver from dimaine silver solution. Introduction to laboratory stains dr chandika epitakaduwa mb bscolombo,d path,mdhistopathology acting. Gastrointestinal and hepatic pathology hepatic vascular diseases hepatic vasculature with the parenchyma, vascular changes can be seen in essentially every type of hepatic disorder. Differential acid dye staining methods generally fall into three distinct groups. Pdf files contains the procedure for a special stain, a procedure card, and sample container labels for the reagents. Use of reticulin stain in the diagnosis of intrauterine.
The findings from wilkins and colleagues are important because diagnosis of prefibrotic myelofibrosis has been controversial and has generated much discussion among those interested in mpds. Brief communication journal of diagnostic pathology. It is found in the basement membrane of many organs i. Used for the identification of reticulin fibers in tissue samples. Reticular fibers crosslink to form a fine meshwork reticulin. It is used to visualize reticular fiber and used extensively in liver histo pathology. In 2001, the world health organization who published criteria for diagnosing mpds and included the concept of prefibrotic myelofibrosis. Reticulin stain is a commonly used special stain to.
A liver biopsy stained using the reticulin demonstrating the normal hepatic plate thickness and mild steatosis. Determining the grade of reticulin fibrosis in the bone marrow is currently performed subjectively using manual and visual grade assignment by hematopathologists with unavoidable intra and interobserver bias. The silver is then reduced with a solution of formaldehyde. When a stain is complete the section is covered with a coverglass that makes the preparation permanent. Grocott methenamine silver stain is preferred for fungi because the periodic acidschiff stain tends not to stain two common lung pathogens. Sign in to save searches and organize your favorite content. Pdf exploratory study of special stains for enhancing histological. Reticulin is a type iii collagen which provides structural integrity. You may do so in any reasonable manner, but not in any. By colouring otherwise transparent tissue sections, these stains allow highly trained pathologists and researchers to view, under a microscope, tissue morphology structure or to look for the presence or prevalence of particular cell types, structures or even microorganisms such as. A modified, inexpensive method for performing the reticulin stain.
Automated grading of reticulin stain of bone marrow trephine using autoretic algorithm h cualing. These manuals are in adobe acrobat pdf format and require the acrobat reader for viewing. Jan 01, 2008 right reticulin stain highlights reticulin fibrosis with reticulin surrounding megakaryocytes. The relevance of reticulin stainmeasured fibrosis at. Conns biological stains, 9th edition williams and wilkins.
Pdf the objective of this study is to investigate the effect of refrigeration on the silver solution prepared for reticulin demonstration. In pathology, the reticulin stain, is a popular staining method in histology. Reticulin stain demonstrates fibers around groups of granulosa cells, while surrounding. Identify and visualize reticular fibers in tissue sections with the thermo scientific richardallan scientific reticulin silver stain, a gordon and sweets modification.
Replace solutions after 1012 slides have been processed, one slide at a time. This file contains additional information such as exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. This kit is easy to use with simplified procedures and delivers stable and improved staining quality. Histopathology special stain reticulin test for surgical. The relevance of reticulin stainmeasured fibrosis at diagnosis in chronic myelogenous leukemia. The reticulin content may be increased or is the defining feature of various disease processes. This is always done as part of the staining process. The loss of normal reticulin staining can help support a diagnosis of hepatocellular carcinoma, and this stain has proven to be particularly helpful on limited biopsies and fineneedle aspirates. While it is not clear in rodent toxicity or preclinical studies if there is a need to distinguish among fibrosis types, much has been recently learned in humans regarding this topic. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. Reticulin stain on bone marrow biopsy shows grade 3 of 3 reticulin fibrosis. Although collagen myelofibrosis indicates poor prognosis in late stages of chronic myelogenous leukemia, the significance of reticulin stain measured fibrosis in newly.
View details of cost of test, pretest information and report availability on dr lal pathlabs. Increased reticulin deposition can be demonstrated by special stains in a patient with a myelodysplastic syndrome accompanied by fibrosis. May 15, 1987 the relevance of reticulin stain measured fibrosis at diagnosis in chronic myelogenous leukemia. This silver staining process is known as silver impregnation. The reticulin stain kit is a metal impregnation technique used to demonstrate reticulin fibres in tissue sections. The reticulin and collagen fiber content of bm is graded semiquantitatively. In humans, increased reticulin fibers are associated with many benign and malignant conditions, while. Pdf recent advances in the pathology of pituitary adenomas. View in gallery flow cytometry shows a large population of mature b cells expressing cd45 a, cd20 and cd38 b, and moderatedensity. Automated grading of reticulin stain of bone marrow trephine. Alveolar filling by neutrophils indicates an acute process, and the leading entity in the differential diagnosis is acute bacterial pneumonia fig. Leukemic diffuse large bcell lymphoma in a patient with. However, the principal staining technique and the gold standard used in histological diagnosis is the haematoxylin and eosin. This network acts as a supporting mesh in soft tissues such as liver, bone marrow, and the tissues and organs of the lymphatic system.
Haematoxylin this is a natural product that is extracted from the heartwood of the logwood tree haematoxylon campechianum which is native to central america and the west indies. The underlying principles have been extensively discussed by bakerbaker 1958. Primary myelofibrosis pmf is a clonal myeloproliferative disorder mpd characterized by a proliferation of abnormal megakaryocytes, reactive bone marrow fibrosis, and extramedullary hematopoiesis see figure. Reticulin stains are commonly used in surgical pathology to assess mass lesions for the possibility of hepatocellular carcinoma. The reticulin kit serves to visualize its existence through an impregnation with a silver salt. Demonstrates the reticular fibers in cirrhosis the fibers are disrupted. Carcinomas have reticulin around clusters of cells. The usefulness of the reticulin stain in the differential diagnosis of liver nodules on fineneedle aspiration biopsy cell block preparations. We report for the first time, to our best knowledge, tacrolimus therapy associated with bone marrow fibrosis, abnormal megakaryocytosis, and dyserythopoiesis in a 17yearold male treated with tacrolimus for nephrotic syndrome. The first staining step is dewaxing which uses a solvent to remove the wax from the slide prior to staining. Gordon and sweets reticulin histotechnology group dst project. Dekmezian r, kantarjian hm, keating mj, talpaz m, mccredie kb, freireich ej. Before ihc, reticulin was used to differentiate sarcomas from carcinomas.
Reticulin is an especially helpful tool in distinguishing small adenomas from normal gland fig. Onestep methods, in which the dyes and other ingredients are combined in a single solution. Hito reticulin optimstain kit offers a simple solution to these problems. Automated grading of reticulin stain of bone marrow. Reticular fibers, reticular fibres or reticulin is a type of fiber in connective tissue composed of type iii collagen secreted by reticular cells. Reticulin fibers are agyrophilic, meaning that these tissue elements will stain black with a silver solution using the aid of a chemical reducer, which brings the silver into a visible form. Most studies have shown that absent or decreased reticulin stain or an abnormal reticulin pattern with widened trabeculae is reliable for the diagnosis of welldifferentiated hcc. Arachnoid hyperplasia is a meningeal response associated with gliomas of. Used for the identification of reticulin fiber in tissue sections, most commonly the liver, kidney and spleen. Hito reticulin optimstain kit is designed based on gordon and sweet method with nuclear counterstain, used to demonstrate reticular fibers.
This kit is extensively used in liver histopathology. Hexose sugars of reticulin demonstrated by oxidation to aldehydes. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Gram stains for bacteria, as well as acidfast and grocott methenamine silver stains for mycobacteria and fungi, respectively, are needed to evaluate for an infectious etiology. Uses gomoris reticulin silver stain reticulin crowding focal hepatocyte loss reticulin collapse hepatocye necrosis thickening hepatic cell plates hepatic regeneration 8. Pdf files contains the information regarding the use and preparation of a working solution. Overview background reticulin purpose of staining types of reticulin stain procedure for gomoris reticulin silver stain uses of gomoris. Reticulin can therefore be useful in the workup of pituitary adenoma, since the basic definition of adenoma rests on the fact that there is disruption and loss of normal acinar architecture. These tumors are characteristically positive for inhibin andor calretinin. The usefulness of the reticulin stain in the differential. Pdf files contains the procedure plus a sample container label.
In order to improve contrast, staining the sections with dyes imparts colour to both cells and tissues. Gomoris silver impregnation staining technique for reticulin. However, these reticulin staining procedures use relatively large volume of staining solutions, are costly, timeconsuming, and difficult to achieve consistent results. Familial pituitary apoplexy as the only presentation of a. Reticulin stain kit with gold chloride from atom scientific. In a study of solitary necrotizing granulomas, elzammar and katzenstein 23 noted that in cases of tuberculosis as well as histoplasmosis the organisms.